Immobilized Recombinant Rat E-Cadherin supports the adhesion of MCF-7 cells in a dose dependent manner. ED 50 for this effect is 0.3 – 1.2 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. Stability testing for Rat E-Cadherin. Rat E-Cadherin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL.

225

Rat E-Cadherin, amino acid Gln24-Ser704 (Accession # NM_031334.1) with a C-terminal human IgG Fc tag, was expressed in 293E cells. Molecular Mass The 939 amino acid recombinant protein has a predicted molecular mass of approximately 104.8 kD. The DTT

The location of cadherin-11 was observed by immunofluorescence microscopy, and its expression was detected by real-time PCR and Western blot. Cell–Cell Interaction Mediated by Cadherin-11 Directly Regulates the Differentiation of Mesenchymal Cells Into the Cells of the Osteo-Lineage and the Chondro-Lineage Isao Kii, 1Norio Amizuka,2,3 Junko Shimomura,2,3 Yumiko Saga,4 and Akira Kudo ABSTRACT: We studied cadherin-11 function in the differentiation of mesenchymal cells. Teratomas P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. 43 ve-cadherin Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation.

  1. Vintersolen stod blott som ett svagt sken
  2. Forsta jobb efter studenten
  3. Mail till skatteverkets inläsningscentral
  4. Lagerchef uppgifter
  5. Bedomningsanvisningar nationella prov svenska

1000 12558 Ensembl ENSG00000170558 ENSMUSG00000024304 UniProt P19022 P15116 RefSeq (mRNA) NM_001308176 NM_001792 NM_007664 RefSeq (protein) NP_001295105 NP_001783 NP_001783.2 NP_031690 Location (UCSC) Chr 18: 27.93 – 28.18 Mb Chr 18: 16.59 – 16.81 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse N-cadherin, also known as Cadherin-2 (CDH2) or neural cadherin (NCAD) is a protein Immunocytochemistry. N‑Cadherin in A549 Human Cell Line. N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. Rabbit polyclonal VE Cadherin antibody - Intercellular Junction Marker.

1A). the total amount of E-cadherin expressed in animals subjected to diversion of the fecal stream for 6, 12, and 18 weeks was reduced in segments without a fecal stream. Cadmium disrupts the cadherin/beta-catenin complex in rat kidney cells, this effect leads to partial activation of beta-catenin-mediated gene transcription.

Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat CDH3 in cell culture supernatants and serum.. Sensitivity: 5pg/ml .

Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species.

Osteo cadherin rat

Immunohistochemistry. N‑Cadherin in Human Heart N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006).

the total amount of E-cadherin expressed in animals subjected to diversion of the fecal stream for 6, 12, and 18 weeks was reduced in segments without a fecal stream. Cadmium disrupts the cadherin/beta-catenin complex in rat kidney cells, this effect leads to partial activation of beta-catenin-mediated gene transcription. SFs separated from rat temporomandibular joint (TMJ) were treated with hydrostatic pressures (HP) of 30, 60, 90, and 120 kPa, as well as tumor necrosis factor-α (TNF-α) for 12, 24, 48, and 72 h. The location of cadherin-11 was observed by immunofluorescence microscopy, and its expression was detected by real-time PCR and Western blot.

Osteo cadherin rat

ZERO BIAS - scores, article reviews, protocol conditions and more Knockout Tested Rabbit recombinant monoclonal N Cadherin antibody [EPR1791-4]. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 92 publication(s). To establish the rat model of alveolar bone osteoporosis, thirty ten-week-old molecules including adhesion molecules and cytokines, such as cadherin-6/2,  2 Nov 2015 free diffusing N-cadherin peptide is supplemented in the osteo- genic media.
Orf programm

Osteo cadherin rat

catalog: 147302. rat monoclonal (DECMA-1) reactivity: human, mouse, dogs. application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section.

rat Cadherin, N- (neural) Cdh2 antibody validated for Immunohistochemistry,Western Blot Javascript is disabled on your browser.
Epost umu

Osteo cadherin rat fylla pa ac goteborg
sfl lagen om förvaltning av samfälligheter
thomas jönsson olofströms kommun
randstad rekryteringskonsult
välkommen tillbaka till skolan
skolgatan 1 a

2017-01-09

Format: 96-well plate with removable strips. 2021-02-03 In order to determine whether E-cadherin is associated with the severity of AP, a pancreatitic rat model was established and the expression levels of E‑cadherin were detected. A study population of 24 Sprague Dawley rats was administered intraperitoneal injections of various concentrations of L‑arginine in order to induce pancreatitis. N-cadherin induced the expression of osteo- and chondro-lineage markers ALP and fibroblast growth factor (FGF) receptor 2.